A single-use, stirred tank bioreactor designed for the non-GMP cultivation of mammalian cell lines. Its rigid design includes a semi-hemispherical shaped polycarbonate vessel, a multi-port high-density polyethylene (HDPE) headplate, a scoping marine impeller, both open pipe and sintered spargers, weldable C-Flex tubing, and all necessary gas inlet and outlet filters. Because the vessel is molded component, additional ports were added below the minimum working volume level including, one luer actuated sample port, one liquid inlet/outlet port, two liquid addition lines, direct sparging lines and a harvest line. All gas and liquid addition ports located below the minimum working volume incorporate check valves to ensure uni-directional flow of gas or liquid. The CellReady 3L Bioreactor is preassembled, gamma irradiated and shipped ready to use.
Specifications
Volume:
Total 3.0 LMinimum 1.0 L
Maximum Working 2.4 L
Dimensions
Vessel diameter (inner) 5.3 (137.0 mm)
Overall height 9.6 (249.0 mm)
Overall weight 1.5 lb (680.0 g)
Base diameter 9.5 (241.0 mm)
Thermowell diameter 0.4 (7.6 mm)
Impeller diameter
(Marine; Scoping)
3.0 (76.2 mm)
Materials
Vessel, shaft and base Polycarbonate Headplate, thermowell and impeller
HDPE
O-rings and seals Silicone
Check valve Silicone, Polypropylene
Septum ABS, Polyisoprene
Probe port plug TPE
Ports
PG 13.5 threaded (3) Fits standard 12 mm probes
(200-235 mm length)
Gas Filters
Overlay and sparge inlets Millipore Millex
33 mm
0.22 m PVDF (hydrophobic)
Vent outlet Millipore 50 mm, 0.22 m
nylon (hydrophobic)
Sampling
Luer actuated valve
Weldable tube with clamp
Sparging
Sintered Polyethylene
Microsparger (pore size)
The inserted frit has been designed to produce a 15-30 micron nominal pore size
Open pipe sparger diameter 0.09 (2.3 mm)
Compatibility
Motor Drive Adapter required Integrates with most standard 3 L motors
Heating blankets
Compatible with most standard 3 L heating blankets
- Please call for details
Connections
C-Flex Clear 374 Tubing ID
Location 1/8 in. (3.2 mm) 1/4 in. (6.4 mm) Connector Filter Component
Fluid addition 1 Headplate yes
1/8 Male
Luer with Cap
Septum
Fluid addition 2 Headplate yes yes
Fluid addition 3 Vessel yes Check valve
Fluid addition 4 Vessel yes Check valve
Harvest Vessel yes yes Clamp
Fluid Addition or Outlet Line Vessel yes Clamp
Air inlet (overlay) Headplate yes
yes
Sparge 1
(micro-sparger) Vessel yes Check valve
Sparge 2
(open pipe) Vessel yes Check valve
Air outlet (vent) Headplate yes yes
Additional Prep Fees May Apply
