The ZOOM IEF Fractionator enriches low abundance proteins and increases the dynamic range of detection. Solution phase isoelectric focusing with the ZOOM IEF Fractionator provides reproducible separations in three hours. Fractionated samples are ready for further analysis by two-dimensional gel electrophoresis (2DE), one-dimensional gel electrophoresis (1DE), or two-dimensional liquid chromatography/mass spectrometry (2D LC/MS).
Built for safety, reliability, and durability
Includes easy-to-assemble sample chambers, in-built buffer chambers, buffer trough, removable electrode assembly, and safety lid
Use with ZOOM Disks and ZOOM Reagents
Sample Chamber and Fractionator Unit Set-up Procedure
The sample chamber and components fit together easily and offer a leak-proof seal
Simply place disks between the sample chambers in the ZOOM IEF Fractionator to allow separation in a specific pH range; for example, to fractionate proteins between pH 4.6 and 5.4, flank a sample chamber with a ZOOM Disk of pH 4.6 and one of pH 5.4
Downstream Processing by 2D Gel Electrophoresis
Following fractionation, the separated samples can be subjected to further analysis using narrow or broad range ZOOM Strips on the ZOOM IPGRunner System
Follow with second dimension analysis using neutral pH NuPAGE ZOOM Gels
Applications:
Isoelectric Focusing, Protein Gel Electrophoresis, Protein Sample Fractionation, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis
Additional Prep Fees May Apply.
