Bio Rad Chef Mapper XA Pulse Field Gel Electrophoresis System (PFGE) with Cooling Module & Variable Speed Pump
***This unit was tested as far as we could. Units power on without errors. All units are preowned with slight wear from normal use. Buyer can expect everything pictured with 30-day warranty. See photos for details.
(Please be advised that any warranty/guarantee/return policy provided from the seller is between the buyer and the seller only. Bid on Equipment is not and cannot be held responsible in any way. Please reference our Terms and Conditions for further information.)
Includes:
Chef Mapper Power Module
Electrophoresis Cell
Cooling Module
Variable Speed Pump
The CHEF MapperXA system is a pulsed field gel electrophoersis (PFGE) system that incorporates patented FIGE and AFIGE* technologies for superior resolution in the range of 100 bp to 10 Mb, in addition to the features of the CHEF-DRII and the CHEF-DR III systems. The CHEF Mapper XA system also includes algorithms for deriving separation conditions. This system is ideal for both the PFGE novice and the seasoned expert.
Automation
The CHEF Mapper XA system offers two ways to optimize your separations. The unique auto-algorithm can automatically select optimal separation conditions, integrating 11 key variables and implementing starting separation conditions. Protocols can be refined using the Windows operating system-based interactive algorithm, which lets you specify several run variables simultaneously to derive optimal separation protocols.
Customization
Busy laboratories need equipment that can store and readily access key separation conditions. The CHEF Mapper XA system can store up to 99 programs.
Application Versatility
The CHEF Mapper XA system is the most flexible of the PFGE units. The CHEF Mapper XA system achieves higher resolution with greater speed and accuracy than any other PFGE system, making it ideal for all PFGE applications. The CHEF Mapper XA system lets you choose any pulse angle from 0 to 360, which allows optimal separation of both chromosomal and plasmid DNA with one system. Accurate sizing of fragments requires an expanded linear range of separation. Switch-time ramps increase the mobility of fragments by gradually changing the switch times during the course of a run. Nonlinear (for example, concave or convex) ramps change the switch-time increments from the start to the end of a run. These nonlinear ramps separate fragments linearly from 50 to 700 kb, yielding more precise fragment size measurements. The multistate mode of the CHEF Mapper XA system enhances resolution in selected fragment size ranges. Each vector (angle of pulse) can be assigned its own voltage (field intensity) and its own switch time (duration of pulse). Up to eight different states can be combined into one run to optimize the separation of subsets of fragments in the sample. The application of secondary pulses of defined voltage, duration, angle, and frequency can enhance the separation and resolution of very large DNA molecules by releasing DNA caught in the gel matrix.
Specifications
Power Module
Dimensions (W x D x H) 55.9 x 34.5 x 30 cm
Construction Aluminum chassis
Weight 16 kg (35.3 lb)
Power supply 350 V maximum, to allow maximum gradient of 9 V/cm, continuously adjustable; built in
Maximum current 0.5 A
Allowable voltage gradients 0.69 V/cm, in 0.1 V/cm increments
Battery back-up All parameters in memory, up to 3 hr of interruption
Delayed start Up to 72 hr
Electrode potentials Dynamically regulated (feedback adjustment) +/- 0.5% F.S.
Program storage 20 average size protocols
Display Fluorescent, 2 lines x 40 characters per line, alphanumeric
Environmental
Operating temperature 50F (10C) to 90F (32C); 3080% humidity
Storage temperature 32F (0C) to 140F (60C); 1090% humidity
Switching Functions
Switching range 50 ms to 18 hr
Switch angle variable 0360 (all electronic switching) in 0.5 increments
Multistate vector switching Up to 15 vectors per pulse cycle, each definable by angle, voltage, and duration
Switch time ramps Linear, concave, or convex using hyperbolic function
Secondary pulses Defined by voltage, frequency, angle, and duration
Field inversion (FIGE) Available with asymmetric forward, reverse voltages
Maximum program blocks 8, with automatic execution
Maximum run time 999 hr per block
Fuses 3 A Slow-Blow; two each for AC line input
Electrophoresis Cell
Dimensions (W x D x H) 44.2 x 50.3 x 11.4 cm, horizontal format
Construction Cover: vacuum-formed polycarbonate, base: injection-molded polycarbonate
Lid Safety interlocked
Weight 10.2 kg (22.5 lb)
Electrodes 24, platinum (0.02 diameter)
Temperature monitoring Via precision temperature probe mounted in base of cell
Additional Prep Fees May Apply
