GE AKTA Pure 25 L Chromatography System 29018224
***Unit is preowned with slight wear from normal use. AKTA Pure 25 L was tested as far as we could. We are unable to fully test this instrument due to, (we don't have the software to fully test this unit.) 30-day warranty included with purchase. See photos for details.
(Please be advised that any warranty/guarantee/return policy provided from the seller is between the buyer and the seller only. Bid on Equipment is not and cannot be held responsible in any way. Please reference our Terms and Conditions for further information.)
Includes:
AKTA Pure 25 L 29018224
Fraction Collector F9-R 29011362
AKTA pure chromatography system - offering a new option to meet your purification needs
AKTA pure is a flexible and intuitive chromatography system for fast purification of proteins, peptides, and nucleic acids from microgram levels to tens of grams of target product. AKTA pure chromatography system is a reliable system where hardware and UNICORN software are designed to work together with columns and chromatography resins to meet purification challenges.
Introducing AKTA pure the first dual wavelength, flexible chromatography system
With 2 fixed wavelengths at 260 and 280 nm AKTA pure offers a new way of addressing your complex purification needs. AKTA pure chromatography systems are available in 25 mL/min and 150 mL/min formats. It retains the flexibility and additional module selection options as AKTA pure L and M. If you have an existing AKTA pure L chromatography system, it can be upgraded with the U9-T UV monitor to perform a broader set of applications.
AKTA pure chromatography system is a highly versatile, modular system with a number of design features to facilitate reliable purification.
AKTA pure chromatography system provides you with the following benefits:
Modular system design with a large range of options to allow flexibility in purification of proteins and peptides
Intuitive and flexible method creation, system control, and evaluation with UNICORN software
Practical size, for easy placement on laboratory bench or in cold cabinet
Reliable system with components and integrated features based on the proven design of AKTA protein purification systems
Predefined method settings for all our laboratory-scale chromatography columns
AKTA pure is a flexible entry system that can be used for a wide variety of fast purifications whether it be peptides, proteins, and nucleic acids from microgram. The AKTA pure FPLC system can be setup to meet your exact needs through is upgradeable platform and has various options from valves and tubing to monitors and fraction collectors. The AKTA Pure is a reliable system built on a long history of FPLC systems installed worldwide.
Parameter AKTA pure 25 L
Application Flexible and intuitive chromatography system for fast purification of proteins and antibodies from microgram to gram scale.
Application supported Affinity chromatography, size exclusion chromatography (SEC, also known as gel filtration), ion exchange chromatography, hydrophobic interaction chromatography, and reversed phase chromatography (RPC). For RPC runs use only Frac F9-R or F9-T.
Automatic buffer preparation No
Autosampler injection No (optional)
Buffer selection Upgradeable to 14 inlets
Compliance The product fulfills valid directives and standards when used within the conditions specified in the user manual. The product must also be used in the same state as it was delivered from Cytiva.
Depth 470 mm
Flow cell path length 2 mm (optional 5 mm)
Flow rate 0.001 to 25 mL/min (normal range), 0.01 to 50 mL/min (column packing flow)
Height 629 mm
Multiple sample injection Upgradeable to 5 samples
Multiple wavelength detection Fixed wavelength
Multistep purification Yes, (optional)
Operating pressure (sample pump) max. N/A
Operating pressure max. 20 MPa (2900 psi)
Power consumption 300 VA
Protection class IP 21
Pump type Piston pump, metering type
Relative humidity 20% to 95% (noncondensing)
Safety certifications CE, ETL, UKCA, EAC, KC, RCM
Tubing i.d. (flow path) 0.5 mm
UV wavelength 280 nm
Viscosity 0.35 to 10.0 cP (system pump)
Voltage 100 to 120/220 to 240 VAC
Weight Up to 53kg
Width 535mm
pH monitoring 0 to 14
pH stability, cleaning-in-place (CIP) 0 to 14
pH stability, operational 2 to 12
AKTA pure can be equipped with up to two fraction collectors, two units of fraction collector F9-R or one F9-R and one F9-C. AKTA go can be used with one fraction collector F9-R. AKTA avant can use one fraction collector F9-R as a second fraction collector in addition the build-in fraction collector.
Collect fractions in 3, 8, 15, and 50 mL tubes.
DropSync function can be used for flow rates up to 2 mL/min to minimizes spillage between drops.
Fraction collection can be based on time, volume, or automatic peak recognition and is controlled from UNICORN software.
Automatic peak recognition minimizes cross-contamination and unwanted eluent can be diverted to the waste.
Parameter Fraction Collector F9-R
Application Affinity Chromatography, Gel Filtration/ Size Exclusion Chromatography, Ion Exchange Chromatography , Hydrophobic Interaction Chromatography and Reversed Phase Chromatography
Wetted Materials Polyetheretherketone (PEEK)
Wetted Part Yes
Pack size 1
Additional Prep Fees May Apply
