Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader
***Unit was tested to specifications. SpectraMax M2 initalizes upons startup and able to reach set point temperature. Instrument is preowned with minimal wear from normal use. 30-day warranty included with purchase. See photos for details.
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The SpectraMax M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. These robust readers have been placed in labs from Antarctica to the International Space Station. With optimized reagents, validation tools, and industry-leading SoftMax Pro Software, they provide consistent performance.
The SpectraMax M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays.
Multi-detection capability
Top- and bottom-read (M2e only)
Dual-mode cuvette port
Dual monochromators
PathCheck and well volume sensor
Instrument and software validation
Robot compatible
SpectraMax M2 and M2e Microplate Readers are the standard for UV/visible multimode reader absorbance, providing ultrafast and full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates
For fluorescence intensity assays, the SpectraMax M2 and M2e units feature an optical design that provides the highest level of flexibility. Users can select from top- or bottom-read modes for improved sensitivity for solution and cell-based assays (M2e only). Assays can be better optimized by scanning across a range of wavelengths in increments as small as 1nm. Up to 4 wavelength pairs can be read in one protocol for endpoint and kinetic measurements, allowing for fast setup of FRET assays.
Applications
DNA/RNA/protein quantitation and purity
PicoGreen/NanoOrange/Bradford
ELISAs/enzyme kinetics (i.e., Km, Ki, etc.)
Drug dissolution profiles
Live/Dead Viability/cytotoxicity assays
Caspase-3 and protease assays
cAMP assays using CatchPoint Assay Kits
Low volume applications
Technical specifications
Absorbance photometric performance
Wavelength selection: Monochromator, tunable in 1 nm increments
Wavelength range: 2001000 nm
Wavelength bandwidth: 4.0 nm
Wavelength accuracy: 2.0 nm
Wavelength repeatability: 0.2 nm
Photometric range: 04.0 OD
Photometric resolution: 0.001 OD
Photometric accuracy (microplate): < 0.006 OD 1.0%, 02 OD
Photometric accuracy (cuvette): < 0.005 OD 1.0%, 02 OD
Photometric precision: < 0.003 OD 1.0%, 02 OD
Baseline flatness: < 0.001 OD
Stray light: < 0.05% @ 230 nm
Fluorescence photometric performance
Dual monochromators: 1 nm increment selection
EX Wavelength range: 250850 nm
EM Wavelength range: M2: 360850 nm, M2e: 250850 nm
Wavelength bandwidth (EX, EM): 9 nm
Top-read optimized sensitivity: 5 pM (fluorescein) in 96 wells
Top-read guaranteed* sensitivity: 15 pM (fluorescein) in 96 wells
Bottom-read optimized sensitivity (M2e only): 10 pM (fluorescein) in 96 wells
Bottom-read guaranteed* sensitivity (M2e only): 25 pM (fluorescein) in 96 wells
Time-Resolved Fluorescence (secondary mode)
EX Wavelength range: 250850 nm
EM Wavelength range: M2: 360850 nm, M2e: 250850 nm
Data collection: 501450 sec., 200 sec. increments
Guaranteed sensitivity*: 7 pM Eu-chelate
Luminescence (secondary mode)
Wavelength range (M2): 360850 nm
Wavelength range (M2e):250850 nm
Guaranteed sensitivity*: 50 fM alkaline phosphatase
General photometric performance
Plate formats: 6, 12, 24, 48, 96, 384 wells
Light source: Xenon flash lamp (1 joule/flash)
Detector: Photomultiplier tube (PMT)
Read time**
96 wells: Abs 18 sec., FI 15 sec.
384 wells: Abs 49 sec., FI 45 sec.
Shaker time: 0 to 999 seconds
Temperature control: Ambient +4C to 45C
Temperature uniformity: < 1C at 37C set point
Temperature accuracy: 1C at 37C set point
Using patented PathCheck PathLength Measurement Technology, these units transform each well in a microplate into a fixed optical pathlength cuvette. They can eliminate standard curves by allowing the calculation of concentrations directly from the absorbance with a known extinction coefficient, as exemplified with nucleic acid and protein quantitation.
Additional Prep Fees May Apply
