Lonza 4D-Nucleofector Core/X unit System
**System unit has been tested to specifications. Has slight cosmetic blemishes from normal use. Buyer can expect a working unit with 30-Day warranty. See photos for further details
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4D-Nucleofector Core with X unit System
The Nucleofector Technology relies on the combination of a Nucleofector Device and cell specific Nucleofector. The Nucleofector Device delivers unique electrical parameters. The electrical settings are pre-programmed for each optimized cell type and can be selected via the device or PC software. We offer three different device platforms plus an add-on device.
Reliable and reproducible transfection technology
Suited for a wide variety of transfection applications
High flexibility with use of different X, Y and LV units
Over 160 optimized protocols and transfection kits available
For transfection of cell lines and primary cells
The 4D-Nucleofector System was developed to offer advanced performance, flexibility and convenience for cell transfection purposes. The system has a modular architecture that allows seamless expansion of the system: it comprises a base core unit and three different functional units to suit your application of interest.
The Nucleofector System allows for transfection of difficult-to-transfect cell lines, as well as a wide variety of primary cells. Cells can be transfected in suspension, or in adherent state with the Y-unit. The electrical parameters of the Nucleofector System are optimized to gain high transfection efficiency (up to 90%) and retain high viability. Cell functionality has been shown to be conserved.
The X Unit of the 4D-Nucleofector System can handle two different Nucleocuvette Vessels both composed out of the same conductive polymer electrode material:
Single 100 L Nucleocuvette Vessels:
Novel conductive polymer 100 L cuvettes replacing former aluminum cuvettes
For high cell numbers at low throughput (e.g. for biochemical applications or Western Blots)
16-well 20 L Nucleocuvette Strips
Same strips as those assembled to a 96-well Nucleocuvette Plate
For low cell numbers at medium throughput (e.g. reporter gene assays, RNAi)
Alternative version available for adherent Nucleofection
Same Conditions for Different Cells Numbers
As the same electrode material is now used for 20 and 100 L cuvettes, Nucleofection Conditions are transferable between the different Nucleocuvette vessels offering maximum flexibility and convenience:
Once the conditions are known for one format they can be easily transferred to the other format.
Conditions are transferable between different throughput formats (4D-Nucleofector System, 96-well Shuttle Device and HT Nucleofector System).
Existing 96-well Shuttle Protocols can be used with the 4D-Nucleofector system.
4D-Nucleofector Core Unit: the controller
Any of the units below (X, Y, LV) can be combined with the Nucleofector Core Unit. The Core Unit controls the functional units of the 4D-Nucleofector System and is equipped with:
5.7 foldable touch screen to operate the system and controlling the functional units
Intuitive operation software for designing and saving individual experimental setups
USB port for software update and data transfer
The X-Unit: transfection of various cell types in various formats
The 4D-Nucleofector X-Unit is the most flexible unit and supports nucleofection of various cell numbers in different formats, as it is equipped with a strip position (16-well strip format) and 2 cuvette positions. This allows you to be flexible in the transfection of your cell type of interest, using a smaller volume or a higher volume.
Features positions for 20l Nucleo cuvette strips (for low cell numbers down to 2 x 10^4) and 100 l single Nucleo cuvette (for cell numbers up to 2 x 10^7)
Different Nucleofection Vessels allow for flexible throughput from 1 to 16 samples
Seamless transfer of conditions between different Nucleofection Vessels: same protocol can be used for use of the strips and the cuvettes.
Comprises HV connectivity for the 96-well Shuttle Device
Overview About Nucleofection Platforms
Device: 4D-Nucleofector System
Throughput (samples per run): Low to medium (1-16)
Reaction volume: 20 L + 100 L
Electrode material: Conductive polymer
Aluminum Low cell numbers (20ul): 2 x 104 to 1 x 106
siRNA amount/sample
(concentration 2 nM 2 M)
0.2 200 pmol (100 L)
0.04 40 pmol (20 L)
High cell numbers (100 L): 2 x 105 to 2 x 107
DNA Vector amount/sample:
0.2 1 g (20 L)
1 5 g (100 L)
Power Conection: 100-240 Vac, 50/60 Hz
Temperature Conditions: 15-40 Deg. C Temperature ambient
Single 100 L Nucleocuvette Vessels: Novel conductive polymer 100 L cuvettes replacing former aluminum cuvettes
For high cell numbers at low throughput
16-well 20 L Nucleocuvette Strips: Same strips as those assembled to a 96-well Nucleocuvette Plate
For low cell numbers at medium throughput (e.g. reporter gene assays, RNAi)
Alternative version available for adherent Nucleofection
Additional Prep Fees May Apply
