Nexcelom Bioscience Cellometer Auto 2000 Cell Profiler Counter
**Unit has been tested for basic functionality. Has slight cosmetic blemishes from normal use. See photos for further details.
The Cellometer Auto 2000 has been particularly optimized to analyze primary cells from bone marrow, cord blood, peripheral blood, and other complex samples for use in various research areas, such as:
Suspensions of tumor cells for oncology
Stem cells for cellular therapy
Splenocytes for development of vaccines
PBMCs for immunology
Nucleated cells for transplantation
Both live and dead nucleated cells can be stained with dual-color fluorescence. This produces precise viability results even in the presence of red blood cells, platelets, and debris. Precise analysis of both clean and messy samples allows the Cellometer Auto 2000 to assess samples at many different points throughout sample processing, right from initial collection through to separation and cryopreservation.
The Cellometer Auto 2000 includes one-touch assays for inspecting a variety of primary samples, such as:
The Cellometer Auto 2000 cell viability counter uses dual-fluorescence imaging and bright-field imaging to rapidly and precisely detect and count individual cells. Count, diameter, concentration, and % viability of cells are calculated and reported automatically. Simple, Automated Cell Counting in 30 Seconds.
Load sample, view image, count cells, and obtain results in less than 30 seconds.
The Auto 2000 enables users to:
Enhance consistency
Increase precision
Increase throughput
Avoid judgment errors, interference from red blood cells, miscounts, and user-to-user variability
Count complex cells (both irregular-shaped and clumpy)
Guarantee that all data is properly captured
-Nucleated Cells from Whole Blood(Immune Cells, high RBC)
-Stem Cells(BR/Green/Red, Stem Cells)
-PBMCs/Splenocytes Following Separation(Immune Cells,Low RBC)
-Tumor and Tissue Cell Suspension(Primary Cells, Cell Lines)
No Interference from Red Blood Cells, Platelets, or Debris
The dual-fluorescence AO/PI technique uses nuclear staining dyes that adhere to nucleic acids in the cell nucleus. Since a majority of the mature mammalian red blood cells lack nuclei, only live mononuclear cells and dead mononuclear cells create a fluorescent signal. This means, red blood cells do not have to be lysed, which saves time and prevents the additional sample preparation step. Debris, platelets, and red blood cells are not counted in the fluorescent channels.
The Advantage of Fluorescent Counting For Primary Cells
The following images reveal the benefit of fluorescent counting for primary cells. The bright-field image demonstrates the combination of platelets, red blood cells, and nucleated cells present in the sample. Only the live nucleated cells and dead nucleated cells are observed and counted in the red and green fluorescent channels.
Fresh human peripheral blood mononuclear cells (PBMCs) were spiked with different amounts of red blood cells. All cells (both RBC and nucleated) were counted in the bright-field channel. Subsequently, nucleated cells were counted in the green fluorescent channel. Different amounts of red blood cells (1.8%, 4.6%, and 8.9%) did not have any impact on the nucleated cell count.
User-Friendly Touch Screen
The easy-to-use touch screen shows pre-optimized assays for normal types of cells. User-defined protocols can be easily produced and saved to the menu. The pre-optimized and simple assays make it easy to operate the instrument and streamline training for novice users.
Cell Size Analysis and Size-Based Counting; It takes about 5 minutes to count 1 x 106 cells with a manual hemacytometer. At times, it takes twice the time to count live cells and dead cells. The Cellometer Auto 2000 Cell Viability Counter estimates the cell count and concentration for both dead and live cells and also calculates % viability in only 30 seconds.
Cellometer Precision
The Cellometer Auto 2000 cell counter provides exceptional reproducibility, with a % coefficient of variation (CV) of less than 10% for fluorescent concentration as well as viability measurements.
The following data is based on four preparations of Jurkat cells stained with a fluorescent nuclear-staining dye called propidium iodide.
The Cellometer Disposable Imaging Chambers contains a pair of separately enclosed chambers with an accurately controlled height. A 20 L cell suspension is placed into the chamber using a regular single-channel pipette. This chamber, in turn, is embedded into the Cellometer cell counter and the cells are finally imaged.
This method of sample loading and analysis is simple and suitable for fragile cells.
The disposable Cellometer cell counting chambers provides many major benefits:
Risk of cross-contamination is eliminated
The washing step is eliminated, which saves time
A controlled volume of samples is achieved
Decreased biohazard risk to users
The most affordable automated counting consumables
Large-depth chambers for huge cells
Additional Prep Fees May Apply
