Agilent 8453 UV-visible Spectroscopy System
**Unit has been tested for basic functionality. VG - There are some minor cosmetic scratches on the sides of the unit... See photos for details
The Agilent 8453 spectrophotometer is a single-beam, microprocessor-controlled, UV-visible spectrophotometer with collimating optics. With its diode-array technology, the spectrophotometer is much faster than comparable conventional instruments with more precision, more sensitivity and more reproducible results. Accessories include special cell holders, peristaltic pumps, autosampler, a multicell transport, a Peltier temperature control accessory, and valve unit and valve-pump controller.
The spectrophotometer can be either controlled from HP and non-HP personal computers equipped with Agilent ChemStation software for UV-visible spectroscopy (personal computers used should be certified by UL1950), or from the dedicated handheld controller that comes with the Agilent 8453E UV-vis spectroscopy system
Featuring exceptional ruggedness and reliability, this Agilent spectrophotometer is easy to use with a line power indicator, a status indicator and other push buttons. This equipment uses a photodiode array (PDA) which highly reliable. Simultaneous measurements of the complete light spectrum take less than a second using this array. Effective data analysis and archival with the use of data analysis software ensures compliance with good laboratory practices (GLP) and pharmacopoeia requirements.
Offering minimum maintenance and an open sample area, the Agilent 8453 Diode Array Spectrophotometer has excellent optical performance. Unlike conventional instruments, the Agilent 8453 is not weighed down by ambient stray light.
Instantly measure sample absorbance across the complete mid-UV-visible spectrum.
Typical scanning spectrophotometers give the user a choice: speed or breadth. Opting for speed, you focus on a particular wavelength of interest (e.g., 280nm for protein concentration, 600nm for cell count), blind to the rest of the spectrum and the interesting and useful data it might contain. Opting for breadth, scanning the entire spectrum can take half a minute or more, often too slow to accurately study a dynamic sample.
With its photodiode-array-based sensor system and only a single moving part, the 8453 simultaneously reads the entire available spectrum, combining speed and breadth with high accuracy and sensitivity. Not only can you look at the absorbance of your sample at standard measurement wavelengths, you can check for unexpected absorbance elsewhere along the spectrum so as to discern impurities, contaminants, and other aberrations.
Spectra are collected in 0.1 seconds, with a resulting sampling frequency limit of 10 Hz, allowing constant and systematic monitoring of samples; particularly useful for dynamic samples
Wavelength range: 190-850nm
Sample must be in solution
Peltier unit attached to instrument for precise temperature control
Open sample compartment for quick sample changing, mixing, reagent addition, etc.
Uses a tungsten lamp for visible wavelengths and a deuterium lamp for UV
Because the diffraction grating is opposite the sample from the light sources, stray light entering the sample compartment is not an issue
Additional Prep Fees May Apply
