Bio Rad Protean i12 IEF Cell Isoelectric Focusing Protein System
***This instrument has been tested to factory specifications. Buyer can expect a working machine and 30-day warranty. Unit has slight wear from normal use. See photos for details.
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The PROTEAN i12 IEF system is designed for isoelectric focusing (IEF) proteins in immobilized pH gradient (IPG) strips for the first dimension of two-dimensional (2-D) electrophoresis. The PROTEAN i12 IEF cell can run 112 IPG strips in 7, 11, 13, 17, 18, or 24 cm focusing trays. Each channel in the isoelectric focusing tray is independently powered, enabling precise control over each IPG strip. This feature makes it possible to run different sample types, different pH gradients, and multiple protocols simultaneously.
Features and Benefits
Optimize experiments in fewer runs the PROTEAN i12 IEF system has the unique ability to run channels under multiple conditions in a single run. Different samples, protocols, and pH gradients can be run simultaneously.
Obtain higher-quality data with less experimental risk each channel is independent, so one irregular sample can no longer compromise the entire run.
Easy, intuitive programming easily run, create, and edit isoelectric focusing protocols from the touch screen user interface. Preprogrammed protocols stored in the internal memory serve as a convenient starting point for developing optimized sample specific IEF conditions.
Run gel side up, gel side down, or with loading cups focusing trays and electrode assemblies accommodate all possible gel configurations.
Applications and Uses
Isoelectric focusing is the first dimension of 2-D electrophoresis, a powerful technique used for the visualization and identification of proteins. The PROTEAN i12 IEF system can be used for a number of applications including the following:
2-D electrophoresis and analysis
Protein profiling analysis of complex protein mixtures
Biomarker discovery/identification of specific targets and markers
Functional proteomics structure, interactions, and function
Manufacturer's Specifications:
Input power 100240 VAC, 50/60 Hz
Fuse Two 6.3-amp, time delay, 5 20 mm
Power input IEC 60320 standard cord set with ground.
Power output
Voltage per lane 0, 50 10,000 V
Current per lane 0100 A, 1 A intervals
Power 0 1 W per lane
Peltier platform
Tray capacity 1 tray
Temperature 1025C 0.5C @ max. ambient temperature 23C
1525C 0.5C @ max. ambient temperature 31C
Focusing trays
Material Polycarbonate
IPG strip length 7, 11, 13, 17, 18, and 24 cm
Capacity 112 IPG strips per focusing tray.
Maximum 7 cm: 7 ml; 11 cm: 10 ml; 13 cm: 11.2 ml.
channel volume 17 cm: 14.2 ml; 18 cm: 15.2 ml.
24 cm: 20.2 ml
Rehydration/equilibration trays
Material Polystyrene
IPG strip length 7, 11, 13, 17, 18, and 24 cm
Capacity 112 IPG strips per tray
Maximum 7 cm: 6.8 ml; 11 cm: 9.6 ml; 13 cm: 10.5 ml.
channel volume 17 cm: 14.2 ml: 18 cm: 16 ml; 24 cm: 19 ml
Environmental requirements for indoor use only, at altitudes of up to 1829 m (6000 ft). Operates at 1035C ambient temperature, with maximum 90% relative humidity.
User Interface
Display QVGA resolution (320 240)
Touch screen or mouse control
Programmable Yes
Ramping
Step, linear, gradual, and hold voltage ramping used for each focusing step.
Hold mode as a final step to prevent diffusion when focusing is complete.
Protocol capacity 2 GB, storage of approximately 20,000 data and protocol files
Data collection .DAT file format
Additional Prep Fees May Apply
