Waters Acquity Classic UPLC System
Includes:
Binary Solvent Manager P/N 186015001
Sample Manager P/N 186015006
Column Heater P/N 186015010
PDA Detector P/N 186015026
The Waters ACQUITY UPLC system provides you with reproducible, dependable and data rich results for challenging separations. This UPLC system features a wide range of flow rates, back pressures and linear velocities, with increased throughput with high efficiency so you get the most for your time and resources.
This ultra-performance liquid chromatography system has a unique sub 2 m particle system that offers 3 times the efficiency of a 5 m system with 70% higher resolution. This higher resolution means more information from more samples in less time. The Waters UPLC system can then run separations at a flow rate 3 times faster than HPLC, without a loss in resolution. This self-contained Waters ACQUITY system contains a PDA Detector, sampler manager autosampler, a binary solvent manager, as well as a solvent and waste tray.
Binary solvent manager
The binary solvent manager is a high-pressure pump that moves solvent through the system. It provides steady (pulse-free) solvent flow at analytical flow rates. The binary solvent manager delivers solvent at flow rates of 1 mL/min at 103,421 kPa (1034 bar, 15,000 psi) and up to 2 mL/min at reduced pressures to 62,053 kPa (621 bar, 9000 psi). The binary solvent manager can pump two solvents simultaneously.
Sample manager
The ACQUITY UPLC sample manager injects the samples it draws from microtiter plates or vials onto the chromatographic column. A locating mechanism uses a probe to access sample locations and draw sample from them. In the needle overfill load-ahead mode, the sample manager can perform an injection in approximately 15 seconds. The first injection requires additional overhead time.
The sample manager accepts standard ANSI/SBS footprint plates, 5.03 0.02 inch 3.365 0.02 inch, that conform to ANSI standards (maximum height = 2.2 inches, including covers). You can program any combination of these plates and vial holders for automated sample processing. Samples are loaded into the sample manager via the front door or the optional sample organizer, which transfers samples back and forth between the two instruments. The sample manager can maintain samples at any temperature between 4 and 40 C (39.2 to 104 F) in 25 C (77 F) or less ambient conditions.
PDA detector
The PDA (photodiode array) optical detector is an ultraviolet/visible light (UV/Vis) spectrophotometer that operates between 190 and 500 nm. The detector offers two flow cell options. The analytical flow cell, with a volume of 500 nanoliters and a pathlength of 10 mm, and the high sensitivity flow cell, with a volume 2.4 microliters and a 25 mm pathlength, both utilize the Waters patented light-guiding flow cell technology.
High temperature column heater
The high temperature (HT) column heater is modular, and its footprint is identical to that of the sample manager. The column heaters front compartment can accommodate any Waters column up to 4.6 mm ID and 150 mm long. The column rests in a U-shaped tray that swivels outward to receive the column from either side. The high temperature column heater heats the column compartment to any temperature from 5 C (9 F) above ambient to 90 C (194 F). A film element insulated to minimize power consumption and facilitate thermal stability is attached to the tray and produces heat. A passive column stabilizer, inside the tray, reduces sensitivity to ambient temperature swings and minimizes bandspreading.
Manufacturer's Specifications:
Solvent Manager
Number of solvents
Up to four, in any combination of two:
A1 or A2 and B1 or B2.
Solvent conditioning
Vacuum degassing (six-channel): one channel per solvent, and two channels for sample manager wash solvents.
Settable flow rate range
0.001 to 2.000 mL/min, in 0.001 mL increments
Gradient profiles
Eleven gradient curves [including linear, step (2), concave (4), and convex (4)] Wet prime Automatic.
Maximum operating pressure
103,421 kPa (1034 bar, 15,000 psi) up to 1 L/min, 62,053 kPa (621 bar, 9000 psi) up to 2 mL/min
Composition accuracy
0.5% absolute (full scale) from 5 to 95% from 0.50 to 2.00 mL/mina
Composition precision
0.15% RSD or 0.04 min SD
Flow accuracy
1.0% at 0.5 mL/min
Sample Manager
Number of SBS/ANSI sample plates
Total of two plates, expandable to up to 22 plates with optional sample organizer.
Maximum of 22 microtiter plates, 11 intermediate depth plates, or 8 deep-well (or 2 mL vial) plates:
96 and 384 microtiter plates
48-position 2 mL vial plates
48-position 0.65 mL micro-centrifuge tube plates
24-position 1.5 mL micro-centrifuge tube plates
Maximum sample capacity
768 in two 384-well plates; expandable to up to 8,448 samples with optional sample organizer
Injection volume range
0.1 to 50 L, in 0.1 L increments,
partial or full loop mode, 10 L loop is.
standard; 1, 2, 5, 20, and 50 L loops
Sample temperature control
4 to 40 C, programmable in 0.1 C increments
Minimum sample required.
5 L residual volume at a needle offset of 0, or 22 L at the preset 2 mm needle offset.
Column Heater
Columns accepted.
Any up to 4.6 mm ID and 150mm long
Temperature range
5 above ambient to 65C (9 above ambient to 194F)
PDA Detector
Wavelength Range
190-500 nm
Flow Cell Options
Analytical Cell 500 nanoliters, 10 mm pathlength
High Sensitivity Cell 2.4 microliters, 25mm pathlength
Additional Prep Fees May Apply
