BIO RAD QX200 AutoDG Droplet Digital PCR System
**This unit has been tested to specifications. Has slight cosmetic blemishes from normal use. Software not included. Buyer can expect same unit as pictures. See photos for further details
The QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules for EvaGreen and probe-based Droplet Digital PCR (ddPCR) applications. The Automated Droplet Generator simplifies the ddPCR workflow, making digital PCR both scalable and practical. The AutoDG Instrument eliminates user-to-user variability, allowing you to fully exploit the precision of ddPCR technology by providing more consistent droplet counts across users and plates. The AutoDG Instrument also minimizes hands-on time it takes less than 5 minutes to load the instrument, and no supervision or user activity is required during droplet generation.
Features and Benefits of the QX200 AutoDG Droplet Digital PCR System
Automated Droplet Generator simplifies the ddPCR workflow and offers flexibility:
Generates droplets for 96 ddPCR reactions in under 45 minutes
Compatible with both the QX100 andQX200 Droplet Digital PCR Systems
A single AutoDG Instrument can supply 45 Droplet Readers continuously
Guides setup and loading with large, color touch-screen interface and LED lighting
User-definable plate layout with increments of 8 wells provides run-to-run flexibility
HEPA-filtered enclosure reduces the possibility of contamination and allows the system to be placed in any lab environment
Droplet Digital PCR Technology the most precise and sensitive digital PCR solution for a wide variety of applications, providing:
Flexible digital PCR chemistry optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
Nanoliter droplet partitioning reduces bias from amplification efficiency differences and PCR inhibitors
Convenient assay design standard curves are not required
Applications of the QX200 Droplet Digital PCR System
Cancer biomarker studies and copy number variation measure varying degrees of cancer mutations, detect rare DNA target copies, and resolve copy number variation states with superior sensitivity and resolution. Predesigned, wet-lab validatedPrimePCR ddPCR Assays are now available for mutation and copy number detection.
Pathogen detection employ the extreme precision of the QX200 System to quantify small fold changes in target DNA or RNA molecules in pathogen detection and monitoring
Next generation sequencing perform absolute quantification of an NGS library without the use of a standard curve
Gene expression analysis achieve reliable and reproducible measurements of small fold changes for low-abundance mRNA and miRNA
Environmental monitoring test a wide variety of environmental samples such as soil and water
Food testing perform routine evaluation of genetically modified organisms (GMO) using validated ddPCR methods
The QX200 AutoDG Droplet Digital PCR System requires the use of the PTC Tempo Deepwell Thermal Cycler orC1000 Touch Thermal Cycler with 96Deep Well Reaction Module and thePX1 PCR Plate Sealer.
Required Consumables
DG32 Cartridges for AutoDG
Automated Droplet Generation Oil for Probes
Automated Droplet Generation Oil for EvaGreen
Pipet Tips for AutoDG
Pipet Tip Waste Bin for AutoDG
Droplet Reader OilorddPCR Droplet Reader Oil EcoTank for Probes
ddPCR Reagents for EvaGreen or TaqMan Hydrolysis Probe-Based Applications
PrimePCR ddPCR Assays
ddPCR Supermixes for Probes (no dUTP)
QX200 ddPCR EvaGreen Supermix
ddPCR Supermix for Probes
One-Step RT-ddPCR Kit for Probes
The AutoDG Instrument partitions each sample in a 96-well PCR plate into ~20,000 uniform nanoliter-sized droplets in which nucleic acid molecules are randomly distributed. Every droplet serves as an individual PCR reaction in Bio-Rads Droplet Digital (ddPCR) PCR System. After partitioning, the plate is sealed with the PX1 Plate Sealer, and PCR is run to end point in a thermal cycler. The droplets in each sample can then be analyzed using a QX600 or QX200 Droplet Reader.
Eliminates user-to-user variability creates more consistent droplet counts across users and plates, enabling you to fully exploit the precision of your ddPCR System
Minimizes hands-on time takes less than 5 minutes to load the instrument, and droplet generation requires no supervision
Features and Benefits of the Automated Droplet Generator
Bio-Rads Automated Droplet Generator simplifies ddPCR workflow and makes digital PCR scalable and flexible:
Guides setup and loading with large, color touch-screen interface and LED lighting
Generates droplets for 96 ddPCR reactions in under 45 minutes
One AutoDG Instrument can generate sufficient droplets to keep 45 droplet readers running continuously
Provides operational flexibility:
User-definable plate layout with increments of 8 wells
HEPA-filtered enclosure reduces the possibility of contamination and allows the system to be placed in any lab environment
Compatible with both QX600 and QX200 ddPCR workflows
Specifications
Starting sample size, l20
Automated Droplet Generator capacity: 196 samples/run
Droplets per 20 l sample: 20,000
QX200 Droplet Reader capacity: 196 samples
Sample illumination: Light-emitting diodes
Sample detection: Multi-pixel photon counter
Detection channels: FAM (EvaGreen), HEX (VIC)
Linear dynamic range: 5 orders of magnitude
Precision: 10%
Droplets per 96-well plate, million: ~1.5
Additional Prep Fees May Apply
